Based on guanidine thiocyanate and phenol, ACExtract Total RNA Extraction Reagent possesses extremely strong lysis capability, can lyse cell and tissue samples at short notice and timely protect the integrity of RNA. It is widely applicable to culture cells, animal tissues, microorganisms, and plant tissues with less frequent secondary metabolism, such as seedling, spire, etc. After thorough lysis of samples in RNA extraction reagent, add chloroform and centrifuge, then the liquor will form a supernatant layer, a middle layer and an organic layer (scarlet lower layer). Collect the supernatant layer which contains RNA and treat with isopropanol precipitation to get total RNA.
Figure : Extract total RNA from mouse heart (15 mg), brain (16 mg), spleen (15 mg), liver (13 mg) and 2x106 HEK293 cells. Elution volume : 100 μl. RNA input : 1-5 μl.