Primary Antibody

AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

  • Catalog Number : A20251PI
  • Number :
  • Size:
    Qty :
  • Price : Request Inquiry
  • print

General Information

Reactivity Human, Mouse, Rabbit, Monkey, Procine
Application WB, IF, IHC, ELISA
Host Rabbit
Uniprot Q13131/P54646
Immunogen The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Formula Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Dilution:

IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Figure:

Western Blot analysis of mouse-lung cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).

Western Blot analysis of various cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1:500

Western Blot analysis of KB cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1:500

The picture was kindly provided by our customer

The picture was kindly provided by our customer

Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of rat-heart tissue.

1,AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).

2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).

3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AMPK alpha (Phospho-Thr172) Antibody

Recently Viewed Products

gotop link
Customized Product Contact Us
0