Antibody, Antibody Sampler Kit

Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit

  • Catalog Number : AK0249
  • Number : AK0249
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Introduction

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c. Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok; and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate. Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis. While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles. Bad is a pro-apoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-xL. Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK and mitochondria-anchored PKA.

General Information

Reactivity Human, Mouse, Rabbit
Application WB, ELISA
Clonality Polyclonal
Conjugate HRP,Non-conjugated
Storage instruction Store at -20℃. Avoid freeze / thaw cycles.
Research topic Regulation of Apoptosis

Product Includes

Product name Quantity  Applications Reactivity Host
BAD Polyclonal Antibody 20μL WB,IHC,ELISA Human, Mouse, Rat Rabbit
Phospho-BAD (Ser112) Polyclonal Antibody 20μL WB,IHC,ELISA Human, Mouse, Rat Rabbit
BAX Polyclonal Antibody 20μL WB,IF,ELISA Human, Mouse, Rat Rabbit
Bim Polyclonal Antibody 20μL WB,IHC,ELISA Human, Mouse, Rat, Monkey Rabbit
BID Polyclonal Antibody 20μL WB,IHC,ELISA Human, Mouse Rabbit
BAK1 Polyclonal Antibody 20μL WB,IHC,IF,ELISA Human, Mouse Rabbit
BBC3 Polyclonal Antibody 20μL WB,ELISA Human,Mouse,Rat Rabbit
Goat Anti-Rabbit IgG (H+L)(peroxidase/HRP conjugated) 120μL WB,ELISA    

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