The partial E gene coding sequence were cloned into retroviral vector by chemical synthesis to obtain FNV-SARS-CoV-2-E pseudovirus. The pseudovirus are prepared with using 293T. For the concentration and purification of the ultracentrifugatio. For the FNV-SARS-CoV-2-E pseudovirus we used ultracentrifuge. Viral envelope of the FNV-SARS-CoV-2-E pseudovirus include the partial E gene and coding sequence, using in experiments which are related to RNA extraction and become the positive control of qPCR.
|Formula||>1 x 107 copy/ml in 1 ml|
|Storage buffer||glucose、KH2PO4、Na2HPO4、NaCl、KCl、FNV-SARS-CoV-2-E pseudovirus|
|Storage instruction||-20°C for 6 months|