Cell culture, Culture Medium, MEMα

MEMα (without phenol red)

  • Catalog Number : CM2509
  • Number : CM2509
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Introduction

MEMα is a versatile basal medium optimized for the growth of mammalian suspension and adherent cell lines, as well as for DHFR-negative cell selection. This α-modified formulation builds on classic MEM by incorporating critical metabolic enhancers while omitting phenol red to reduce assay background. MEMα supports a broad range of cell types, including keratinocytes, astrocytes, CHO-DG44, and melanoma lines.
Form

Liquid

Cell Line

MSC, CHO, Primary rat astrocytes, Human, keratinocytes, Melanoma cells, NK-92 MI, NK/T, MC3T3-E1

Sterility

Sterile-filtered

Endotoxin

≤0.25 EU/mL

pH

7.0-7.4

Osmolality

280-330 mOsm/kg

Quality

GMP-compliant under the ISO 13485 standard

Serum level

Standrad serum supplementation

Storage conditions

2-8° C. Protect from light

Shelf life

12 months

Modification
With (+) Without (-)
Ribonucleosides Phenol red
Deoxyribonucleosides HEPES
L-Glutamine  
Sodium pyruvate  

 

 

 

Product Highlights
  • Broad Cell Compatibility

    Supports both anchorage-dependent and suspension cultures, including primary cells and transfected DHFR-negative lines (e.g., CHO-DG44).

  • Assay-Ready, Low-Background

    Free of phenol red to minimize interference in spectrophotometric, fluorescence, and luminescence assays.

  • Enhanced Metabolic Support

    Enriched with key cofactors (pyruvate, lipoic acid, vitamins) and stabilized L-glutamine to promote robust cell growth and viability.

  • DHFR Selection Capability

    Optional omission of nucleosides allows direct use as selective medium for DHFR-deficient cell lines.

  • Quality Manufacturing

    Produced under GMP conditions, with USP-grade water for injection to ensure ultra-low endotoxin and RNase/DNase-free performance.

Recommended Use
  • Supplementation

    Protein- and growth factor–free. We recommend adding 10% Fetal Bovine Serum (FBS) or defined serum replacements according to your application needs.

  • Culture Conditions

    Incubate in 5–10% CO₂ at 37 °C to maintain optimal pH with the sodium bicarbonate buffering system.

  • Applications

    • Routine propagation of mammalian cell lines
    • Transfection, gene amplification, and selection studies
    • Primary cell maintenance and stem cell expansion
    • Drug screening, toxicology assays, and bioproduction

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