Introduction
MEMα is a versatile basal medium optimized for the growth of mammalian suspension and adherent cell lines, as well as for DHFR-negative cell selection. This α-modified formulation builds on classic MEM by incorporating critical metabolic enhancers while omitting phenol red to reduce assay background. MEMα supports a broad range of cell types, including keratinocytes, astrocytes, CHO-DG44, and melanoma lines.
| Form | Liquid
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| Cell Line | MSC, CHO, Primary rat astrocytes, Human, keratinocytes, Melanoma cells, NK-92 MI, NK/T, MC3T3-E1
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| Sterility | Sterile-filtered
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| Endotoxin | ≤0.25 EU/mL
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| pH | 7.0-7.4
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| Osmolality | 280-330 mOsm/kg
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| Quality | GMP-compliant under the ISO 13485 standard
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| Serum level | Standrad serum supplementation
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| Storage conditions | 2-8° C. Protect from light
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| Shelf life | 12 months
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| Modification |
| With (+) |
Without (-) |
| Ribonucleosides |
Phenol red |
| Deoxyribonucleosides |
HEPES |
| L-Glutamine |
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| Sodium pyruvate |
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| Product Highlights |
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Broad Cell Compatibility
Supports both anchorage-dependent and suspension cultures, including primary cells and transfected DHFR-negative lines (e.g., CHO-DG44).
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Assay-Ready, Low-Background
Free of phenol red to minimize interference in spectrophotometric, fluorescence, and luminescence assays.
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Enhanced Metabolic Support
Enriched with key cofactors (pyruvate, lipoic acid, vitamins) and stabilized L-glutamine to promote robust cell growth and viability.
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DHFR Selection Capability
Optional omission of nucleosides allows direct use as selective medium for DHFR-deficient cell lines.
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Quality Manufacturing
Produced under GMP conditions, with USP-grade water for injection to ensure ultra-low endotoxin and RNase/DNase-free performance.
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| Recommended Use |
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Supplementation
Protein- and growth factor–free. We recommend adding 10% Fetal Bovine Serum (FBS) or defined serum replacements according to your application needs.
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Culture Conditions
Incubate in 5–10% CO₂ at 37 °C to maintain optimal pH with the sodium bicarbonate buffering system.
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Applications
- Routine propagation of mammalian cell lines
- Transfection, gene amplification, and selection studies
- Primary cell maintenance and stem cell expansion
- Drug screening, toxicology assays, and bioproduction
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