RB1 Antibody

Catalog Number : A20359PI
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General Information

Reactivity	Human, Mouse, Rabbit
Application	WB, IF, IHC, ELISA
Host	Rabbit
Uniprot	P06400
Immunogen	The antiserum was produced against synthesized peptide derived from human Retinoblastoma. AA range:751-800
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Formula	Liquid in PBS containing ₅₀% glycerol, ₀.₅% BSA and ₀.₀₂% sodium azide.
Alias	RB; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Figure:

Immunofluorescence analysis of rat-lung tissue. 1,Rb Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Rb Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,Rb Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

mmunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Rb Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.