Antibody, Antibody Sampler Kit
Fos Family Antibody Sampler Kit
- Catalog Number : AK0161
- Number :
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Size:
Qty : - Price : Request Inquiry
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Introduction
The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids. The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity. Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization. Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells. Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours. FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells. Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells.
General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Storage instruction | Store at -20℃. Avoid freeze / thaw cycles. |
| Research topic | Oncogenes; Cancer research |
PRODUCT INCLUDES
|
Cat No. |
Product name |
Quantity |
Applications |
Reactivity |
Host |
|
A340479 |
c-Fos Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340312 |
Phospho-c-Fos (Ser32) Polyclonal Antibody |
20μL |
WB, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340524 |
FOSB Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Mouse, Monkey |
Rabbit |
|
A340528 |
FOSL1 Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A1013s |
Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) |
120μL |
WB, ELISA |
Rabbit |
Goat |
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