Cell culture, Culture Medium

DMEM/F-12 Dry Powder Media

  • Catalog Number : CM1015、CM1016
  • Number : CM1015
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Introduction

Dulbecco's Modified Eagle Media: Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal Media for supporting the growth of many different mammalian cells. DMEM/F12 Dry Powder Media contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Media. It doesn’t contains proteins, lipids, or growth factors. Serum supplements are required before use, usually with 10% Fetal Bovine Serum (FBS) or Specsure® Bovine Growth Serum. Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH. Cells successfully cultured in DMEM / F-12 include neurons, smooth muscle cells, MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

General Information

Application Cell
Storage instruction 2-8 ˚C
  • Sterility:Sterile-filtered
  • Form:Powder
  • Application (s):Cell culture/ mammalian: suitable
  • Impurities:Endotoxin, tested
  • Components:

    HEPES (+)

    L-glutamine (+)

    D-glucose (3200 mg/ml)

    Phenol red (+)

    Sodium bicarbonate (-)

 

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