COVID-19 , COVID-19 (SARS-CoV-2) Detection kits, COVID-19 Pseudovirus, Pseudovirus, SARS-CoV-2

Pseudovirus-SARS-CoV-2-M

  • Catalog Number : PV023
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Introduction

The partial M gene coding sequence were cloned into retroviral vector by chemical synthesis to obtain FNV-SARS-CoV-2-M pseudovirus. The pseudovirus are prepared with using 293T. For the concentration and purification of the ultracentrifuge. For the FNV-SARS-CoV-2-M pseudovirus we used Ultracentrifuge. Viral envelope of the FNV-SARS-CoV-2-M pseudovirus include the partial M gene and coding sequence, using in experiments which are related to RNA extraction and become the positive control of qPCR.

General Information

Formula >1 x 107 copy/ml in 1 ml
Storage buffer glucose、KH2PO4、Na2HPO4、NaCl、KCl、FNV-SARS-CoV-2-M pseudovirus
Storage instruction -20°C for 6 months

M Gene

GGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAA

AAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTAT

TCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAA

CAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTC

TGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTG

CTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGC

TATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGC

TACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTT

CCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAA

CGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTA

GAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGAC

ATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACAT

CAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGA

ACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAG

CAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGAT

TGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGT

GACAATATTGCTTTGCTTGTACAGTAA

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