COVID-19 , COVID-19 (SARS-CoV-2) Detection kits, COVID-19 Pseudovirus, Pseudovirus, SARS-CoV-2

Pseudovirus-SARS-CoV-2-N

  • Catalog Number : PV024
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Introduction

The partial N gene coding sequence were cloned into retroviral vector by chemical synthesis to obtain FNV-SARS-CoV-2-N pseudovirus. The pseudovirus are prepared with using 293T. For the concentration and purification of the ultracentrifuge. For the FNV-SARS-CoV-2-N pseudovirus we used ultracentrifuge. Viral envelope of the FNV-SARS-CoV-2-N pseudovirus include the partial N gene and coding sequence, using in experiments which are related to RNA extraction and become the positive control of qPCR.

General Information

Formula >1 x 107 copy/ml in 1 ml
Storage buffer glucose、KH2PO4、Na2HPO4、NaCl、KCl、FNV-SARS-CoV-2-N pseudovirus
Storage instruction -20°C for 6 months

N Gene

ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCC

GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAA

CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGT

CGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCA

CCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCC

TCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCA

GATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAA

TTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAG

ATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGA

CTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTG

CAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGG

CACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAA

CTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAG

AAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTC

ATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGC

AGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATG

GCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATT

GAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAA

CAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGG

CTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGC

ATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAA

CAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGAC

AAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATT

TGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATT

GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACA

CAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAA

AGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATAC

AAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGA

AGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAA

GAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTG

GATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTG

CTGACTCAACTCAGGCCTAA

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