.png)
Chemical, Buffer, Western Blotting, Buffer
NuBlot™ Stripping Buffer, 5X
- Catalog Number : A1039
- Number : A1039
-
Size:
Qty : - Price : Request Inquiry
-
Introduction
NuBlot™ Stripping Buffer effectively removes antibodies from Western blots in one minute. The unconjugated antigens on the stripped membrane are allowed to be reprobed and be detected with chemiluminescent substrates. NuBlot™ Stripping Buffer is an ideal product for breaking antigen-antibody interaction, saving time and saving conserving samples.
General Information
| Application | WB, Chemical |
|---|---|
| Storage instruction | NuBlot Stripping Buffer could be stored at room temperature. Expiration date is labeled on the bottle or box. |
Procedure
- Prepare NuBlot™ Stripping Buffer by mixing 1 part of NuBlot™ Stripping Buffer (5X) and 4 parts of ddH2O.
- Wash nitrocellulose or PVDF membrane in wash buffer to remove the chemiluminescent substrate.
- Incubate the membrane in NuBlot™ Stripping Buffer for 1-3 minutes at room temperature while shaking.
- Discard NuBlot™ Stripping Buffer and wash the membrane 3 times in wash buffer.
- Re-block the stripped membrane and perform immunodetection by Western Blot normal protocol.
.png)
.png)