ELISA

Porcine BNP (Brain Natriuretic Peptide) ELISA Kit

  • Catalog Number : E0026
  • Number : E0026
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Introduction

This ELISA kit applies to the in vitro quantitative determination of Porcine BNP concentrations in serum, plasma and other biological fluids.

General Information

Reactivity Porcine
Assay principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine BNP. During the reaction, Porcine BNP in the sample or standard competes with a fixed amount of Porcine BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine BNP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate
well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Porcine BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Sensitivity 18.75 pg/mL
Detection range 31.25-2000 pg/mL
Sample type serum, plasma, cell lysates, tissue homogenates,cell culture supernatant, and other biological fluids.
Storage instruction 4 ˚C or -20˚C for 6 months

CONTENTS and STORAGE

Item

Specifications

Storage

Micro ELISA Plate (Dismountable)

8 wells ×12 strips

-20°C, 6 months

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100×)

1 vial, 120 uL

Concentrated HRP Conjugate (100×)

1 vial, 120 μL

-20°C (Protect from light), 6 months

Reference Standard & Sample Diluent

1 vial, 20 mL

4°C, 6 months

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25×)

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

4°C (Protect from light)

Stop Solution

1 vial, 10 mL

4°C

Plate Sealer

5 pieces

Product Description

1 copy

Certificate of Analysis

1 copy

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