Protein Biology, Protein ladders

AceColor™ prestained Protein marker (1.5-40 kDa)

  • Catalog Number : A1059
  • Number : A1059
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Introduction

ACE Biolabs AceColor™ Prestained Protein Ladder is a mixture of 8 blue-, a orange-, and a green-stained proteins (1.5 to 40 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

General Information

Applications

  • Monitoring protein migration during SDS-PAGE.
  •  Monitoring protein transfer efficiency on membranes after Western blot transferring.
  • Sizing estimation of proteins on SDS-PAGE and Western blots.

 

PROTOCOL

  • For SDS-PAGE: Loading 3 μl or 5 μl per well for clear monitoring electrophoresis on a 15-well
    or 10-well mini-gel. Apply more for a thicker (> 1.5 mm) or larger gel.
  • For Western blotting: Loading 3~5 μl per well for monitoring electrophoresis and general
    Western blot transferring efficiency. Apply more for a thicker (> 1.5 mm) or larger gel.

Note: Do NOT heat, dilute, or add reducing agents before loading.

 

LADDER

Band weights (kD)

1.5/3.5/5/7.5/10/15/20/25/30/40

PRODUCT USE LIMITATION   

These products are intended for research use only.

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