GKLF Mouse mAb
- Catalog Number : AM0308
- Number : AM0308
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General Information
| Reactivity | Human | |||||||||
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| Application | WB, IF, IHC, ELISA | |||||||||
| Host | Mouse | |||||||||
| Clonality | Monoclonal | |||||||||
| Conjugate | Non-conjugated | |||||||||
| Immunogen | Purified recombinant fragment of human GKLF expressed in E. Coli. | |||||||||
| Molecular Weight | 55kD (Calculated) | |||||||||
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | |||||||||
| Storage instruction | -15°C to -25°C/1 year(Do not lower than -25°C) | |||||||||
| Research topic | >>Signaling pathways regulating pluripotency of stem cells>>Chemical carcinogenesis - receptor activation | |||||||||
| Alias | KLF4
EZF GKLF Krueppel-like factor 4 Epithelial zinc finger protein EZF Gut-enriched krueppel-like factor |
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| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. | |||||||||
| Specificity | GKLF Monoclonal Antibody detects endogenous levels of GKLF protein. | |||||||||
| Purification | Affinity purification | |||||||||
| Gene Name | KLF4 | |||||||||
| Protein Name | Krueppel-like factor 4 | |||||||||
| Database Link |
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| Background | This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]. | |||||||||
| Function | Function:Transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interaction with the C-terminal domain of MUC1 enhances suppression of TP53/p53 transcription. | |||||||||
| Cellular Localization | Nucleus. | |||||||||
| Tissue Expression | Cervix,Lung,Placenta,Substantia nigra,Tongue | |||||||||
| Validation Data |
Western Blot analysis using GKLF Monoclonal Antibody against HEK293 (1) and KLF4-hIgGFc transfected HEK293 (2) cell lysate. | |||||||||
Immunohistochemistry analysis of paraffin-embedded colon cancer tissues (left) and lung cancer tissues (right) with DAB staining using GKLF Monoclonal Antibody. | ||||||||||
Immunofluorescence analysis of ECA109 cells using GKLF Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. |
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