Antibody, Primary Antibody

Histone H2AG Antibody

  • Catalog Number : A0519P
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Introduction

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

General Information

Application WB, IF, IHC, ELISA
Clonality Polyclonal
Conjugate Non-conjugation
Uniprot P0C0S8
Isotype IgG
Immunogen Recombinant Human Histone H2A type 1 protein (2-130AA)
Purity >95%, Protein G purified
Storage buffer Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM, H2AFP; H2AFC; H2AFD; H2AFI; H2AFN

 

 

 

 

Western blot All lanes: HIST1H2AG antibody at 4µg/ml + HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa

 

 

 

 

 

Immunohistochemistry of paraffin-embedded human lung cancer using CSBPA010389LA01HU at dilution of 1:100

 

 

 

 

Immunohistochemistry of paraffin-embedded human testis tissue using CSBPA010389LA01HU at dilution of 1:100

 

Chromatin Immunoprecipitation Hela(1.2*106 )were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010389HU) against the PGK1 promoter.

Immunofluorescent analysis of Hela cells using CSB-PA010389LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

 

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