Enzyme, RNA reagent/RT-PCR, qPCR

ACE Script II U+ One Step qRT-PCR Kit (Probe)

  • Catalog Number : EP2027
  • Number : EP2027
  • Size:
    Qty :
  • Price : Request Inquiry
  • Download DataSheet print

Introduction

ACE Script II U+ One Step qRT-PCR Kit (Probe) is designed for quantitative PCR with RNA (such as RNA virus) as templates. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers (GSP), without extra opening pipes and pipetting, thus it increases the quantification throughput and minimizes the chance of contamination. The kit introduces dUTP/UDG anti-contamination system. Heat-labile UDG can degrade contamination with U rapidly at room temperature and will be inactivated at 55℃ during reverse transcription, without affecting efficiency and sensitivity of RT-PCR. Integrating the advantages of ACE Script II Reverse Transcriptase, Champagne Taq DNA Polymerase and optimized buffer system, One Step qRT-PCR Probe Kit enables highly sensitive detection as low as 0.1 pg of total RNA or RNA with less than 10 copies. The kit is provided as convenient Master Mix. 2×One-Step U+ Mix contains optimized buffer, dNTP/dUTP Mix, and detection system for fluorescent probes (such as TaqMan). One-Step U+ Enzyme Mix contains proportion-optimized HiScript II Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Champagne Taq DNA Polymerase.

General Information

Application qPCR, RT
Storage instruction All components should be stored at -20℃.

CONTENTS

Component

EP2027 250 rxn (20 μl/rxn)

RNase free ddH2O

2 × One Step U+ Mix a

One Step U+ Enzyme Mix b

50 × ROX Reference Dye 1 c

50 × ROX Reference Dye 2 c

1.25 ml × 2

1.25 ml × 2

250 µl

100 µl

100 µl

a. Contains dNTP/dUTP Mix and Mg2+

b. Contains HiScript II Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Champagne Taq DNA Polymerase

c. used to calibrate the error of fluorescence signals between different wells. Use 50×ROX Reference Dye 1 for ABI 7900HT/7300 Real-Time PCR System and StepOne PlusTM. Use 50×ROX Reference Dye 2 for ABI 7500, 7500 Fast Real-Time PCR System and Stratagene Mx3000P. The ROX is not needed for Roche or Bio-Rad Real-Time PCR instruments

Recently Viewed Products

gotop link
Customized Product Contact Us
0