Enzyme, RNA reagent/RT-PCR, qPCR
ACE Script II U+ One Step qRT-PCR Kit (Probe)
- Catalog Number : EP2027
- Number : EP2027
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Introduction
ACE Script II U+ One Step qRT-PCR Kit (Probe) is designed for quantitative PCR with RNA (such as RNA virus) as templates. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers (GSP), without extra opening pipes and pipetting, thus it increases the quantification throughput and minimizes the chance of contamination. The kit introduces dUTP/UDG anti-contamination system. Heat-labile UDG can degrade contamination with U rapidly at room temperature and will be inactivated at 55℃ during reverse transcription, without affecting efficiency and sensitivity of RT-PCR. Integrating the advantages of ACE Script II Reverse Transcriptase, Champagne Taq DNA Polymerase and optimized buffer system, One Step qRT-PCR Probe Kit enables highly sensitive detection as low as 0.1 pg of total RNA or RNA with less than 10 copies. The kit is provided as convenient Master Mix. 2×One-Step U+ Mix contains optimized buffer, dNTP/dUTP Mix, and detection system for fluorescent probes (such as TaqMan). One-Step U+ Enzyme Mix contains proportion-optimized HiScript II Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Champagne Taq DNA Polymerase.
General Information
| Application | qPCR, RT |
|---|---|
| Storage instruction | All components should be stored at -20℃. |
CONTENTS
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Component |
EP2027 250 rxn (20 μl/rxn) |
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RNase free ddH2O 2 × One Step U+ Mix a One Step U+ Enzyme Mix b 50 × ROX Reference Dye 1 c 50 × ROX Reference Dye 2 c |
1.25 ml × 2 1.25 ml × 2 250 µl 100 µl 100 µl |
a. Contains dNTP/dUTP Mix and Mg2+
b. Contains HiScript II Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Champagne Taq DNA Polymerase
c. used to calibrate the error of fluorescence signals between different wells. Use 50×ROX Reference Dye 1 for ABI 7900HT/7300 Real-Time PCR System and StepOne PlusTM. Use 50×ROX Reference Dye 2 for ABI 7500, 7500 Fast Real-Time PCR System and Stratagene Mx3000P. The ROX is not needed for Roche or Bio-Rad Real-Time PCR instruments
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