Enzyme, RNA reagent/RT-PCR

ACE Scipt II One Step RT-PCR Kit

  • Catalog Number : EP2006
  • Number : EP2006
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Introduction

The ACE Scipt II One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the ACE Scipt II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, Champagne Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb).

General Information

Application RT
Storage instruction All components should be stored at -20℃.

CONTENTS

 

Component

EP2006 50 rxn (50 μl/rxn)

RNase free ddH2O

2× One Step Mixa

One Step Enzyme Mixb

10× Loading buffer

1 ml × 2

625μl × 2

125μl

1.25 ml

 

a. Contains dNTPs.

b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.

 

 

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