ELISA, Assay Kits

Porcine Platelet-derived growth factor subunit BB(PDGF-BB) ELISA kit

  • Catalog Number : E0023
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Introduction

This kit allows for the determination of PDGF-BB concentrations in Porcine serum, plasma, tissue homogenates and other biological fluids.

General Information

Reactivity Porcine
Application ELISA
Assay principle The kit assay Porcine PDGF-BB level in the sample, use Purified Porcine PDGF-BB antibody to coat microtiter plate wells, make solid-phase antibody, then add PDGF-BB to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At
HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PDGF-BB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sensitivity The minimum detectable dose is typically less than 0.1 ng.
Detection range 0.25 ng/mL - 8 ng/mL
Sample type serum, plasma, Urine, cell culture supernatant, Tissue samples

MATERIALS PROVIDED WITH THE KIT

Materials provided with the kit

48 determinations

96 determinations

Storage

User manual

1

1

Closure plate

membrane

2

2

Sealed bags

1

1

Microelisa stripplate

1

1

2-8

Standard

0.3ml×6 bottle

          0.3ml×6 bottle

2-8

HRP-Conjugate

reagent

5ml×1 bottle

          10ml×1 bottle

2-8

Sample diluent

3ml×1 bottle

          6ml×1 bottle

2-8

             Chromogen

              Solution A

3ml×1 bottle

          6ml×1 bottle

2-8

              Chromogen

               Solution B

3ml×1 bottle

         6ml×1 bottle

2-8

Stop Solution

3ml×1 bottle

          6ml×1 bottle

2-8

20×Wash solution

15ml×1 bottle

          25ml×1 bottle

2-8

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