Porcine Platelet-derived growth factor subunit BB(PDGF-BB) ELISA kit
- Catalog Number : E0023
- Number :
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Size:
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Introduction
This kit allows for the determination of PDGF-BB concentrations in Porcine serum, plasma, tissue homogenates and other biological fluids.
General Information
| Reactivity | Porcine |
|---|---|
| Application | ELISA |
| Assay principle | The kit assay Porcine PDGF-BB level in the sample, use Purified Porcine PDGF-BB antibody to coat microtiter plate wells, make solid-phase antibody, then add PDGF-BB to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At
HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PDGF-BB in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Sensitivity | The minimum detectable dose is typically less than 0.1 ng. |
| Detection range | 0.25 ng/mL - 8 ng/mL |
| Sample type | serum, plasma, Urine, cell culture supernatant, Tissue samples |
MATERIALS PROVIDED WITH THE KIT
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Materials provided with the kit |
48 determinations |
96 determinations |
Storage |
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User manual |
1 |
1 |
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Closure plate membrane |
2 |
2 |
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Sealed bags |
1 |
1 |
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Microelisa stripplate |
1 |
1 |
2-8℃ |
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Standard |
0.3ml×6 bottle |
0.3ml×6 bottle |
2-8℃ |
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HRP-Conjugate reagent |
5ml×1 bottle |
10ml×1 bottle |
2-8℃ |
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Sample diluent |
3ml×1 bottle |
6ml×1 bottle |
2-8℃ |
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Chromogen Solution A |
3ml×1 bottle |
6ml×1 bottle |
2-8℃ |
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Chromogen Solution B |
3ml×1 bottle |
6ml×1 bottle |
2-8℃ |
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Stop Solution |
3ml×1 bottle |
6ml×1 bottle |
2-8℃ |
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20×Wash solution |
15ml×1 bottle |
25ml×1 bottle |
2-8℃ |
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