Chemical

30% Acrylamide Solution (37.5:1)

  • Catalog Number : C1006
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General Information

Application WB
Introduction Acrylamid is prepared from electrophoresis grade acrylamide and bis-acrylamide in Tri-Distilled water The product is passed through a 0.45um filter
Cas NO.  79-06-1
Formula C3H5NO
Molecular Weight 71.08
Caution Acrylamide is a potent neurotoxin and absorbed through the skin. The effects of acrylamide are cumulative. Although polyacrylamide is suspected to be nontoxic, it should be handled with great care because of the possibility that it might contain small quantities of unpolymerized acrylamide Acrylamide.
Storage

4 °C 

Formulation

1. Stacking Gel  

 

Final Acrylamide Conc.

5%

ddH2O

4.2 ml

30% Acrylamide

1 ml

1 M Tris-HCl (pH6.8)

0.76 ml

10% APS

60 μl

10% SDS

60 μl

TEMED

6 μl

Total Volume

6 ml

 

2.  Separating Gel 

 

Final Acrylamide Conc.

6%

8%

10%

12%

15%

ddH2O

5.3 ml

4.65 ml

4 ml

3.3 ml

2.3 ml

30% Acrylamide

2 ml

2.65 ml

3.3 ml

4 ml

5 ml

1.5 M Tris-HCl (pH8.8)

2.5 ml

2.5 ml

2.5 ml

2.5 ml

2.5 ml

10% APS

100 μl

100 μl

100 μl

100 μl

100 μl

10% SDS

100 μl

100 μl

100 μl

100 μl

100 μl

TEMED

7.6 μl

7.6 μl

7.6 μl

7.6 μl

7.6 μl

Total Volume

10 ml

10 ml

10 ml

10 ml

10 ml

 

 

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