Annexin V-FITC/PI Apoptosis Detection Kit
Annexin V is recognized as one of the sensitive indicators of early apoptosis. Apoptosis can be detected by fluorescence microscopy or flow cytometry using Annexin V probe conjugated to FITC (a green fluorescent).This kit also includes propidium iodide (PI), a dye for nucleic acid that cannot penetrate the intact cell membrane of healthy or early apoptotic cells.Using both Annexin V and PI, this kit can used to distinguish cells at different apoptotic periods, i.e. live cells (Annexin V-/PI-), early apoptotic cells (Annexin V+/PI-), and late apoptotic/dead cells (Annexin V+/PI+).
Figure : Flow cytometry analysis of apoptosis stages.
(A)-(E) : Jurkat cells are treated with 0, 0.5, 1.5, 4.5, 13.5 ng/ml TRAIL (TNF-related apoptosis-inducing ligand) for 3 hrs, respectively.
(F) : After treated with 13.5 ng/ml TRAIL for 3 hrs, Jurkat cells are incubated with 10 ug unlabel-Annexin V for 10 mins and then stained with Annexin V-FITC and PI. The signal of right-down shift to left-down quadrant shows that the specificity of Annexin V staining.