Chemical, Inhibitor, Western Blotting, Protein Extraction

100X Protease Inhibitor Cocktail (EDTA-Free)

  • Catalog Number : A1029
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General Information

Application Extraction
Description 

Protease Inhibitor Cocktail protects proteins from degration by proteases during protein extraction procedure and ensure the integrity of protein for further experiment.

Formulation 

100X Liquid in DMSO.

Ingredient Cas No. Target 100X Conc.
AEBSF 30827-99-7 Serine proteases 104 mM
Aprotinin 9087-70-1 Serine proteases 80 μM
Bestatin 58970-76-6 Aminopeptidases 5 mM
E-64 66701-25-5 Cysteine proteases 1.5 mM
Leupeptin 103476-89-7 Serine and Cysteine proteases 2 mM
Pepstatin A 26305-03-3 Aspartic proteases 1.5 mM
Protocol

Before extraction, dilute Protease Inhibitor with 1X RIPA Lysis

Buffer in 1: 100 ( v/v ) ratio. 

Related Product

1X RIPA Lysis Buffer

5X RIPA Lysis Buffer

100X Phosphatase Inhibitor Cocktail (A+B) 

Storage -20 ℃ for 2 years
Alias PI

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