LC3B Polyclonal Antibody

Catalog Number : A20321PI
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General Information

Reactivity	Human, Mouse, Rabbit
Application	WB, IF, IHC
Host	Rabbit
Uniprot	Q9GZQ8
Immunogen	Recombinant Protein of MAP LC3β
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Formula	PBS, pH ₇.₄, containing ₀.₀₂% sodium azide as Preservative and ₅₀% Glycerol.

	Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using MAP LC3β Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
	Immunohistochemical analysis of paraffin-embedded Rat BrainTissue using MAP LC3β Polyclonal Antibody.