Enzyme, RNA reagent/RT-PCR

T7 High Yield RNA Transcription Kit

  • Catalog Number : EP5001
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General Information

Charactistics
  • High yield - Produces 150-200 µg RNA from 0.5 µg template in 20 µl reaction solution.
  • Flexible - Generates unlabeled, biotin-labeled or dye-labeled RNA.
  • Optimized reaction system for in vitro transcription
  • T7 High Yield RNA Transcription Kit contains T7 RNA Polymerase Mix, 10X Reaction Buffer, ATP Solution, UTP Solution, GTP Solution, CTP Solution, DNase I, Control Template (0.5 μg/μl), and Nuclease-free Water.
Figures

Figure 1. Transcription reaction catalyzed by T7 RNA polymerase

Figure 2. Accumulation curve of RNA transcript in standard reaction with 3 different size of templates.

Figure 3. Accumulation of RNA transcript in strandard transcription reaction with different amount of 0.8 kb PCR product as templates after 2 hours incubation.

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