Antibody, Antibody Sampler Kit
Double Strand Breaks (DSB) Repair Antibody Sampler Kit
- Catalog Number : AK0148
- Number :
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Size:
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Introduction
Double strand DNA breaks (DSB) in mammalian cells can be repaired by the related mechanisms of non-homologous end-joining (NHEJ) and homologous recombination (HR). A DNA-dependent protein kinase composed of DNA-binding subunits Ku70 and Ku86 and the DNA-PKcs catalytic subunit mediates NHEJ repair. The Ku heterodimer binds free DNA ends and recruits DNA-PKcs to the break. DNA-PKcs signals areas of DNA damage and recruits additional proteins, such as the Artemis exo- and endonuclease that processes and primes the damaged sequence. Following replacement DNA synthesis, a ligase complex composed of DNA ligase IV and XRCC4 joins the repaired ends. XRCC4-like factor (XLF) is an essential ligase-associated repair factor that promotes gap-filling during NHEJ. Homologous recombination utilizes aligned homologous sequences as a repair template. The MRN complex, composed of Mre11, Rad50, and nibrin (p95/NBS1), plays a critical role in sensing, processing and repairing breaks. MRN interacts with BRCA1 and CtIP to facilitate 5’ resection of DSB DNA to generate 3’ ssDNA ends necessary for repair. DNA-binding protein Mre11 exhibits exonuclease and endonuclease activity and is largely responsible for ssDNA end processing. Interaction between the MRN complex and ATM kinase promotes association between the kinase and its substrates and likely leads to ATM activation. ATM acts a central controller of the cell cycle checkpoint by phosphorylating multiple targets, including c-Abl, BRCA1 and p95/NSB1. Activated c-Abl phosphorylates Rad52, which promotes Rad51 binding to ssDNA and subsequent annealing of ssDNA.
General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, ELISA |
| Host | Rabbit |
| Storage instruction | Store at -20℃. Avoid freeze / thaw cycles. |
| Research topic | DNA damage and repair |
PRODUCT INCLUDES
|
Cat No. |
Product name |
Quantity |
Applications |
Reactivity |
Host |
|
A340385 |
Phospho-ATM (Ser1981) Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse |
Rabbit |
|
A340439 |
ATM Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse |
Rabbit |
|
A340501 |
PRKDC Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Mouse |
Rabbit |
|
A340579 |
XRCC5 Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Monkey |
Rabbit |
|
A340597 |
MRE11 Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat, Monkey |
Rabbit |
|
A340655 |
RAD50 Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A1013s |
Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) |
120μL |
WB, ELISA |
Rabbit |
Goat |
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