Antibody, Antibody Sampler Kit
ER Stress-induced Autophagy Antibody Sampler Kit
- Catalog Number : AK0156
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Introduction
The endoplasmic reticulum (ER) is an organelle with essential biosynthetic and signaling functions in eukaryotic cells. Post synthesis of secretory and transmembrane proteins on polysomes, proteins are translocated into the ER where they are often modified by disulfide bond formation, amino-linked glycosylation, and folding. Different physiological and pathological conditions can disturb proper protein folding in the ER causing ER stress. ER stress activates an intracellular signaling transduction pathway called unfolded protein response (UPR) and autophagy to avoid cell death. The main role of UPR is to improve the protein load on the ER by shutting down protein translation and gene transcription to enhance ER's folding capacity. On the other hand, autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmc contents. One of the chaperones aiding in proper protein folding is Binding immunoglobulin Protein (BiP). BiP works by binding to misfolded proteins to prevent them from forming aggregates and assists in proper refolding. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. One of the proteins critical to autophagy process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30. Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells. Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak. Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the
General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, ELISA |
| Host | Rabbit |
| Storage instruction | Store at -20℃. Avoid freeze / thaw cycles. |
| Research topic | ER proteins; Stress response |
Product Includes
| Product name | Quantity | Applications | Reactivity | Host |
| HSPA5 Polyclonal Antibody | 20μL | WB,IHC,IF,ELISA | Human, Mouse, Rat, Monkey | Rabbit |
| EIF2 alpha Polyclonal Antibody | 20μL | WB,IHC,ELISA | Human, Mouse, Rat, Monkey | Rabbit |
| Phospho-EIF2 alpha (Ser51) Polyclonal Antibody | 20μL | WB,IHC,ELISA | Human, Mouse, Rat | Rabbit |
| BECN1 Polyclonal Antibody | 20μL | WB,ElLISA | Human, Mouse | Rabbit |
| JNK1/2/3 Polyclonal Antibody | 20μL | WB,IHC,IF,ELISA | Human, Mouse, Rat | Rabbit |
| Phospho-JNK1/2/3 (Thr183/Y185) Polyclonal Antibody | 20μL | WB,ELISA | Human, Mouse, Rat | Rabbit |
| Goat Anti-Rabbit IgG (H+L)(peroxidase/HRP conjugated) | 120μL | WB,ELISA |
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