Antibody, Antibody Sampler Kit
Phospho-Erk1/2 Pathway Sampler Kit
- Catalog Number : AK0225
- Number :
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Size:
Qty : - Price : Request 詢價
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Introduction
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.
General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | HRP,Non-conjugated |
| Storage instruction | Store at -20℃. Avoid freeze / thaw cycles. |
| Research topic | MAPK-ERK Signaling Pathway |
PRODUCT INCLUDES
|
Cat No. |
Product name |
Quantity |
Applications |
Reactivity |
Host |
|
A340316 |
Phospho-RAF1 (Ser338) Polyclonal Antibody |
20μL |
WB, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340252 |
Phospho-MAP2K1/2 (Ser218/222) Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340370 |
Phospho-ERK 1/2 (Thr202/Tyr204) Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340299 |
Phospho-RPS6KA1 (Ser380) Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340317 |
Phospho-RPS6KA5 (Ser376) Polyclonal Antibody |
20μL |
WB, IF, ELISA |
Human, Mouse |
Rabbit |
|
A1013s |
Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) |
120μL |
WB, ELISA |
Rabbit |
Goat |
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