His-Tag Monoclonal Antibody
- Catalog Number : A1026M
- Number :
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Size:
Qty : - Price : Request 詢價
- Stock : Request
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General Information
| Application | WB, IF, ELISA, IP |
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| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Non-conjugated |
| Immunogen | Synthetic Peptide |
| Storage buffer | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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Image |
2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000 Lane 1: untransfected 293 cell lysate Lane 2: transfected 293 cell lysate with His-tag fusion protein Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose) Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose) Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose) Lane 6: Recombinant protein (E.coli) Immunofluorescence(IF) 1- His-Tag Monoclonal Antibody IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His) Western Blotting(WB) 1- His-Tag Monoclonal Antibody 2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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