Western Blotting, ECL

ACEsignal Chemiluminescent Substrate - Femto

  • Catalog Number : A1042
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ACEsignal Chemiluminescent Substrate - Femto is an extremly sensitive ECL product by supplementing a proprietary luminol for chemiluminescent detection of immobilized proteins (Western blotting), conjugated with Horseradish Peroxidase (HRP) directly or indirectly. In Western blotting application, ACEsignal Chemiluminescent Substrate - Femto provides high signal and low background, which allows detection of protein targets at femtogram levels. This feature benefits the researchers with excellent low background results and without signal burn effects at the same time.

General Information

Storage instruction ACEsignal Chemiluminescent Substrate - Femto should be stored at 2-8 °C and shielded from light. Expiration date is labeled on t


  1. PVDF or nitrocellulose membrane
  2. Wash buffer: Phosphate-buffered saline (PBS) or Tris-buffered saline (TBS) containing 0.05–0.1% Tween®-20
  1. PBS: 10 mM KH2PO4,150 mM NaCl, pH 7.4
  2. TBS: 25 mM Tris, 150 mM NaCl, pH 7.4
  1. Blocking buffer: 1–5% (w/v) blocking agent (e.g., casein, BSA, or gelatin) in wash buffer
  2. Specific primary antibody for interested protein, diluted in blocking buffer
  3. HRP-conjugated secondary antibody, specific for primary antibody, diluted in blocking buffer
  4. X-ray film or chemiluminescence image acquisition systems


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