COVID-19 , COVID-19 Pseudovirus

Pseudovirus-SARS-CoV-2

  • Catalog Number PV031
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Description ACE Biolabs used the lentiviral packaging system to package the SARS-CoV-2 (2019-nCoV, COVID-19 virus) S protein as a surface capsid glycoprotein and containing GFP and Luciferase dual reporter genes as a pseudovirus model. The virus can infect cell lines overexpressing the ACE2 gene. Researchers can determine the infection efficiency by observing GFP through a microscope and detecting the value of Luciferase.
Assay principle Research use only Recommended amount: 2-10 μl/well of 96-well plate for ACE2 overexpressing cell lines. According to experimental conditions, it can be adjusted. The infection efficiency of pseudoviruses highly depends on cell types. It is recommended to conduct a pre-experiment before the formal experiment to determine the optimal amount of virus.
Concentration > 107 TU/ml (The functional titer is determined by GFP-positive ACE2 overexpressing HEK293T cells.)
Storage instruction The product can be stored at -80°C or below for 6 months. Avoid repeated freezing and thawing cycles.

Construction

Image

HEK293-ACE2-OE cell infected by Pseudovirus-SARS-CoV-2, can be observed the expression of green fluorescent protein and detected the activity of luciferase.

 

NOTE

  1. The pseudovirus-SARS-CoV-2 pseudovirus we provided is a replication-defective virus, that is, the virus does not use the host cell to generate new virus particles after infecting the target cells.
  2. The experimental operation needs to be carried out in the BSL-2 laboratory and Class II biological safety cabinet, and wear personal protective equipment such as laboratory clothes, masks and gloves.
  3. If this product is accidentally spilled during the experiment, please use disinfectant to inactivate it immediately. If it splashes on the eyes, skin or other body parts, immediately rinse with plenty of water.
  4. The experimental waste generated by using this product needs to be autoclaved and treated according to the medical waste treatment requirements.
  5. The infection efficiency of pseudoviruses highly depends on cell types. It is recommended to conduct a pre-experiment before the formal experiment to determine the optimal amount of virus.

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