Introduction
This kit uses Competitive-ELISA as the method. It can detect Aflatoxin B1 (AFB1) in samples, such as grain, formula feed, edible oil, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, AFB1 in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AFB1 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of AFB1. The concentration of AFB1 in the samples can be calculated by comparing the OD of the samples to the standard curve.
General Information
| Application | ELISA |
|---|---|
| Sensitivity | 0.03 ppb (ng/mL) |
| Sample type | Grain,Corn skin, Wheat bran, Edible oil, Peanut,Biscuits, Beer,Wine, Soy sauce, Vinegar |
| Storage instruction | Storage at 4˚C for 1 year. Avoid freeze |
SPECIFICITY
- Sensitivity: 0.03 ppb (ng/mL)
- Reaction mode: 25˚C, 30-15 min
- Detection limit:
Grain---0.15 ppb; Corn skin, Wheat bran---0.6 ppb; Edible oil, Peanut---0.6 ppb,
Biscuits---0.3 ppb; Beer----0.3 ppb; Wine, Soy sauce, Vinegar---0.15 ppb
- Cross-reactivity: Aflatoxin B1 (AFB1) ---100%
- Sample recovery rate:
Grain, Corn skin, Wheat bran, Edible oil---85±15%; Peanut---82±15%;
Biscuits---83%±15%; Beer---84%±15%;
Wine, Soy sauce, Vinegar---87%±15%
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