Enzyme, Restriction Enzyme

Mbo II

  • Catalog Number : RE0471
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General Information

Storage buffer 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.

Recognition site

GAAGA(N)8
CTTCT(N)7

Source

An E.coli strain, that carries the cloned gene Mbo II from Moraxella bovis

Assayed on

Lambda DNA (dam-)

Unit definition

One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.

Optimal SE-buffer

Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

Enzyme activity (%)

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