Cell culture, COVID-19 , COVID-19 Pseudovirus, Cell Line

ACE2-3xFLAG-HEK293T stable cell line

  • Catalog Number : CL0021
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Introduction

ACE2 is also called ACEH and is called angiotensin converting enzyme 2. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidase, and is the functional receptor for SARS and HCoV-NL63 human coronavirus S glycoprotein. This ACE2-3xFLAG-HEK293T stable cell line expresses the ACE2-3xFLAG fusion protein, which can be used for ACE2 gene related research.

General Information

Research topic COVID-19

CONSTRUCTION

 

Stable Cell Line: The ACE2-3xFLAG-HEK293T stable cell line was obtained from lentivirus infected and multiple rounds of Puromycin screening. The lentivirus was packaged with pLV-ACE2-3FLAG plasmid, concentrated and purified, and then infected with HEK293T cells.

 

Vector Information                             

 

APPLICATIONS

ACE2-3xFLAG-HEK293T stable cell infected by Pseudovirus-SARS-CoV-2 (ACE #PV031), can be observed the expression of green fluorescent protein and detected the activity of luciferase.

 

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