COVID-19 , COVID-19 Pseudovirus, Pseudovirus, SARS-CoV-2

Pseudovirus-SARS-ORF1a-N

  • Catalog Number : PV026
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Introduction

Particial of ORF1a/b gene sequences and all of N Gene coding sequence from the Severe Acute Respiratory Syndrome (SARS) were obtained by chemical synthesis and cloned into a retrovirus vector. 293T cells were used for the pseudovirus preparation. The tomography column was used for virus purification following super centrifuge concentration. The envelope protein of pseudovirus is coded by ORF1a/b Gene Sequence, N Gene Coding Region Sequence, which can be used for viral RNA nucleic acid extraction and as positive control products for Q-PCR testing.

General Information

Assay principle Research Recommended amount: 50-100 μl/time. According to experimental conditions, it can be adjusted.
Storage buffer glucose、KH2PO4、Na2HPO4、NaCl、KCl
Storage instruction The product can be stored at -20°C or below for 6 months. Avoid repeated freezing and thawing cycles.

Construction

SEQENCE INFORMATION

1. SARS-ORF1aCCTGCTAAAGCATATAAGGATTACCTAGCAAGTGGAGGACAACCAATCACCAACTGTGTGAAGATGTTGTGTACACACACTGGTACAGGACAGGCAATTACTGTAACACCAGAAGCTAACATGGACCAAGAGTCCTTTGGTGGTGCTTCATGTTGTCTGTATTGTAGATGCCACATTGACCATCCAAATCCTAAAGGATTCTGTGACTTGAAAGGTAAGTACGTCCAAATACCTACCACTTGTGCTAATGACCCAGTGGGTTTTACACTTAGAAACACAGTCTGTACCGTCTGCGGAATGTGGAAAGGTTATGGCTGTAGTTGTGACCAACTCCGCGAACCCTTGATGCAGTCTGCGGATGCATCAACGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTCTACAGGGCTTTTGATATTTACAACGAAAAAGTTGCTGGTTTTGCAAAG

2. SARS-N GeneGCGTCTTGGTTCACAGCTCTCACTCAGCATGGCAAGGAGGAACTTAGATTCCCTCGAGGCCAGGGCGTTCCAATCAACACCAATAGTGGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCCGACGAGTTCGTGGTGGTGACGGCAAAATGAAAGAGCTCAGCCCCAGATGGTACTTCTATTACCTAGGAACTGGCCCAGAAGCTTCACTTCCCTACGGCGCTAACAAAGAAGGCATCGTATGGGTTGCAACTGAGGGAGCCTTGAATACACCCAAAGACCACATTGGCACCCGCAATCCTAATAACAATGCTGCCACCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAGGGAAGCAGAGGCGGCAGTCAAGCCTCTTCTCGCTCCTCATCACGTAGTCGCGGTAATTCAAGAAATTCAACTCCTGGCAGCAGTAGGGGAAATTCTCCTGCTCGAATGGCTAGCGGAGGTGGTGAAACTGCCCTCGCGCTATTGCTGCTAGACAGATTGAACCAGCTTGAGAGCAAAGTTTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCATCTAAAAAGCCTCGCCAAAAACGTAC

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