This product includes ACE Hot Start Taq DNA Polymerse (Dye Plus), dNTPs, and an optimized buffer system that allows for amplification by simply adding primers and templates, reducing pipetting and improving throughput and reproducibility. A protective agent added to the amplification system allows the 2X ACE Hot Start Taq Master Mix (Dye Plus) to maintain stable activity after repeated freeze-thaw cycles. This product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3' end of the PCR product, A, can be directly cloned into the T vector and used in the ACE One Step Cloning Kit series (ACE, Cat.No. #EC1001, #EC1008).