ELISA

β-agonist (Beta-agonist) ELISA Kit

  • Catalog Number : E5021
  • Number : E5021
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General Information

Application ELISA
Assay principle This kit uses Competitive-ELISA as the method. It can detect β-agonist in samples, such as tissues, urine, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, β-agonist in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti- β-agonist antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of β-agonist. The concentration of β-agonist in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity 0.2 ppb (ng/mL)
Storage instruction Store at 2~8℃ for 1 year. Avoid freeze.
Reaction mode 

25℃, 15 min~15 min ~15 min

Detection limit 

Urine --- 1 ppb; Muscle --- 1 ppb

Cross-reactivity 

 

Clenbuterol

100%

Clorprenaline

141%

Tulobuterol

80%

Brombuterol, Mapenterol

107%

Cimbuterol

86.9%

Clenpenterol

78.3%

Terbutaline

60.6%

Salbutamol

60.6%

Mabuterol

56.3%

Bambuterol

18%

Cimaterol

14%

Clenproperol

5%

Ractopamine, Zilpaterol, Phenylethanolamine A

1%

Sample recovery rate 

90%±30%

Kit components 

 

Item

Specifications

ELISA Microtiter Plate

96 wells

Standard Liquid

1 mL each (0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb)

HRP Conjugate

12 mL

Antibody Working Solution

7 mL

Substrate Reagent A

6 mL

Substrate Reagent B

6 mL

Stop Solution

6 mL

Sample Stabilizer

5 mL

Sample Solution

30 mL

20×Concentrated Wash Buffer

25 mL

Plate Sealer

3 pieces

Sealed Bag

1 piece

Manual

1 copy

Note 

All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

Other materials required but not supplied  Instrument: Microplate Reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g). 
Micropipettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL). 
Reagents: Trichloroacetic acid, NaOH

 

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