Protein Biology

Recombinant Human Gamma-glutamyl hydrolase (GGH)

  • Catalog Number : P0054
  • Number : P0054-20ug
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Introduction

Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.

General Information

Reactivity Human
Host E.coli
Uniprot Q92820
Purity >85% (SDS-PAGE)
Storage buffer Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Storage instruction Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alias Conjugase; folylpolygammaglutamyl hydrolase; gamma glutamyl hydrolase; Gamma-Glu-X carboxypeptidase; Gamma-glutamyl hydrolase (conjugase; folylpolygammaglutamyl hydrolase); Gamma-glutamyl hydrolase; GGH; GGH_HUMAN; gH
Physical Appearance 

Lyophilized powder

Target Protein Sequence 

RPHGDT AKKPIIGILM QKCRNKVMKN YGRYYIAASY VKYLESAGAR VVPVRLDLTE KDYEILFKSI NGILFPGGSV DLRRSDYAKV AKIFYNLSIQ SFDDGDYFPV WGTCLGFEEL SLLISGECLL TATDTVDVAM PLNFTGGQLH SRMFQNFPTE LLLSLAVEPL TANFHKWSLS VKNFTMNEKL KKFFNVLTTN TDGKIEFIST MEGYKYPVYG VQWHPEKAPY EWKNLDGISH APNAVKTAFY LAEFFVNEAR KNNHHFKSES EEEKALIYQF SPIYTGNISS FQQCYIFD

Reconstitution 

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

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