RPMI-1640 Media
- Catalog Number : CM1017
- Number : CM1017
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Introduction
RPMI-1640 media is an improved Mccoy's 5A Media, which uses bicarbonate buffer system. RPMI-1640 Media was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. RPMI-1640 Media contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives. RPMI-1640 media doesn’t contain proteins, lipids, or growth factors. Serum supplements are required before use, usually with 10% Fetal Bovine Serum (FBS) or Specsure® Bovine Growth Serum. Uses sodium bicarbonate buffer system (2 g/L), and therefore requires a 5% CO2 environment to maintain physiological pH. Cells successfully cultured in RPMI-1640 media include cancer cell, astrocyte, MCF-7, Jurkat, PC-12, PBMC and HeLa.
General Information
| Application | Cell |
|---|---|
| Storage instruction | Storage at 2-8˚C |
- Sterility：Sterile-filtered
- Form：liquid
- Application (s)：Cell culture/ mammalian: suitable
- Impurities：Endotoxin, tested
- Components：
HEPES (-)
L-glutamine (+)
D-glucose (2000 mg/ml)
Phenol red (+)
Sodium bicarbonate (-) - Storage temp：2-8 ˚C
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