Chemical, Cell culture, Cell staining, Cell staining
DAPI
- Catalog Number : C3315
- Number : C3315
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Introduction
DAPI (4 ', 6-diamidino-2-phenylindole), is a kind of fluorescent dye which can strongly combine with DNA. It can be observed by fluorescence microscope. DAPI can penetrate the normal cell membrane, it can be used for staining of living cells or fixed cells.
DAPI can penetrate the cell membrane and combine with the double stranded DNA in the nucleus and produces more than 20 times stronger fluorescence than DAPI itself. It has stronger sensitivity to double stranded DNA staining than EB. Cells can be seen with blue fluorescence when DNA combine with DPAI by microscope. And the efficiency of cell labeling is high (almost 100%) without any effect on living cells. DAPI staining is often used to detect apoptosis. After staining, it is observed by fluorescence microscope or flow cytometry. DAPI is also commonly used in general nuclear staining and in some specific cases double stranded DNA staining. After heat shock treatment, the cells staining with DAPI for 3 min, and the morphological changes of nuclei could be seen by fluorescence microscope.
The maximum excitation wavelength of DAPI is 340 nm, and the maximum emission wavelength is 488 nm. When DAPI combines with dsDNA, the maximum excitation wavelength is 364 nm, and the maximum emission wavelength is 454 nm.
DAPI can penetrate the cell membrane and combine with the double stranded DNA in the nucleus and produces more than 20 times stronger fluorescence than DAPI itself. It has stronger sensitivity to double stranded DNA staining than EB. Cells can be seen with blue fluorescence when DNA combine with DPAI by microscope. And the efficiency of cell labeling is high (almost 100%) without any effect on living cells. DAPI staining is often used to detect apoptosis. After staining, it is observed by fluorescence microscope or flow cytometry. DAPI is also commonly used in general nuclear staining and in some specific cases double stranded DNA staining. After heat shock treatment, the cells staining with DAPI for 3 min, and the morphological changes of nuclei could be seen by fluorescence microscope.
The maximum excitation wavelength of DAPI is 340 nm, and the maximum emission wavelength is 488 nm. When DAPI combines with dsDNA, the maximum excitation wavelength is 364 nm, and the maximum emission wavelength is 454 nm.
General Information
| Application | FCM, Cell, FC |
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| Cas No. | 28718-90-3 |
| Purity | ≥98% |
| Molecular Weight | 350.25 |
| Formula | C16H15N5．2HCl |
| Solubility | H2O: 10 mg/mL heat or sonication 28718-90-3may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks；PBS: insoluble |
| Storage instruction | Store at -20℃ and potected from light |
| Alias | 4,6-Diamidino-2-phenylindole dihydrochloride, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride |
| Fluorescence |
maximum excitation wavelength λex 340 nm; maximum emission wavelength λem 488 nm (nur DAPI); maximum excitation wavelength λex 364 nm after combination of DAPI and double-stranded DNA; maximum emission wavelength λem 454 nm (DAPI-DNA-Komplex) |
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| Appearance |
Yellow powder |
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