Factor XIIIa (PT1976) mouse mAb
- Catalog Number : A130237
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Introduction
Specificity：
This antibody detects endogenous levels of human Factor XIIIa. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section.
Background：
coagulation factor XIII A chain(F13A1) Homo sapiens This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot.
Subcellular Location：extracellular region,platelet alpha granule lumen,blood microparticle,
This antibody detects endogenous levels of human Factor XIIIa. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section.
Background：
coagulation factor XIII A chain(F13A1) Homo sapiens This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot.
Subcellular Location：extracellular region,platelet alpha granule lumen,blood microparticle,
General Information
| Reactivity | Human |
|---|---|
| Application | IHC |
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Non-conjugated |
| Uniprot | P00488 |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human Factor XIIIa |
| Assay principle | IHC-p 1:100-500 |
| Sample type | paraffin section |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Expression region | Brain,Pancreas,Plasma, |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instruction | -20°C/1 year |
| Research topic | blood coagulation, hemostasis, response to wounding, peptide cross-linking, wound healing, coagulation, regulation of body fluid levels, |
| Alias | F13A1 F13A |
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