Cell culture, Stem Cell

Human Mesenchymal Stem Cell Culture Kit (Serum-Free)

  • Catalog Number : SCK-CM01
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Introduction

Human Mesenchymal Stem Cell Culture Kit (Serum-Free) can be used for primary cell separation and cell passage of human mesenchymal stem cells from various sources, such as bone marrow (BM-hMSC), adipose tissue (AT-hMSC), umbilical cord (UCM-hMSC), etc, while maintaining their potential for multidirectional differentiation.
There is no need to add serum or serum substitutes to use this product. This product has a clear chemical composition and does not contain any animal-derived components.
The difference between product batches is small, and all raw materials comply with GMP standards. More suitable for clinical research purposes.

General Information

Storage instruction 2-8℃ or -20℃, avoid light and repeated freezing and thawing. The prepared complete medium is stored at 2-8℃, and the validity period is 1 week.

MAIN COMPONENTS

Amino acids, vitamins, inorganic salts, human albumin, transferrin, insulin, trace elements, etc. PRODUCT

 

PERFORMANCE INDEX

1. Time required to harvest primary cells from fresh human umbilical cord: 12-14 days

2. The time required for cell passage: 3-4 days (5000cells/cm2).

3. Cell Phenotype: CD34 and CD45 are negatively expressed, and CD44, CD73, CD90 and CD105 are positively expressed.

4. Cell morphology: The cells are spindle-shaped and grow in a fingerprint or spiral shape.

 

PRODUCT SPECIFICATION

Appearance: The basic medium is a pale pink liquid and the additives are a yellow liquid

pH value: 7.0-8.0 at 25℃

Osmotic pressure: 280-320 mOsm/Kg

Endotoxin: <0.5EU/mL

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