Insulin (PT2015) mouse mAb
- Catalog Number : A130250
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Introduction
Specificity：This antibody detects endogenous levels of human Insulin. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section.
Background：insulin(INS) Homo sapiens After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Function：
Disease: Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
Function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Subcellular Location：Cytoplasmic
Background：insulin(INS) Homo sapiens After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
Function：
Disease: Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
Function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Subcellular Location：Cytoplasmic
General Information
| Reactivity | Human |
|---|---|
| Application | IHC |
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Non-conjugated |
| Uniprot | P01308 |
| Isotype | IgG2b, Kappa |
| Immunogen | Synthesized peptide derived from human Insulin |
| Assay principle | IHC-p 1:100-500 |
| Sample type | paraffin section |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Expression region | Blood,Liver,Muscle,Pancreas |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instruction | -20°C/1 year |
| Research topic | MAPKKK cascade, skeletal system development, ossification, regulation of cell growth, osteoblast differentiation, cell activation, regulation of cytokine production, regulation of protein amino acid phosphorylation, positive regulation of protein amino acid phosphorylation, acute inflammatory response, regulation of acute inflammatory response,negative regulation of acute inflammatory response, negative regulation of immune system process, regulation of immune effector process, negative regulation of immune |
| Alias | INS |
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