ACE Biolabs used the lentiviral packaging system to package the SARS-CoV-2 (2019-nCoV, COVID-19 virus) D614G mutation protein as a surface capsid glycoprotein and containing GFP and Luciferase dual reporter genes as a pseudovirus model. The virus can infect cell lines overexpressing the ACE2 gene. Researchers can determine the infection efficiency by observing GFP through a microscope and detecting the value of Luciferase.
|Assay principle||Research use only
Recommended amount: 2-10 μl/well of 96-well plate for ACE2 overexpressing cell lines.
According to experimental conditions, it can be adjusted. The infection efficiency of pseudoviruses highly depends on cell types. It is recommended to conduct a pre-experiment before the formal experiment to determine the optimal amount of virus.
|Storage instruction||The product can be stored at -80°C or below for 6 months. Avoid repeated freezing and thawing cycles.|