Nco I
- Catalog Number : RE0047
- Number :
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General Information
| Storage buffer | Store at -20°C. |
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Product Name |
Nco I |
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Cat NO. |
RE0047/RE0047L |
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Size |
1,000 unit / 5,000 units |
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Concentration |
20,000 units/ml |
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Recognition site |
C↓CATGG
GGTAC↑C |
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Source |
Bacillus species 19 |
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Enzyme activity (%) |
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Reaction Conditions |
1X Buffer Y, BSA (100 μg/ml). Incubate at 37 oC |
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1X SE-Buffer Y |
Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
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Heat Inactivation |
Enzyme is inactivated by incubation at for 20 minutes. |
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Optimal temperature |
37oC |
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Unit Definition |
One unit is defined as the amount of enzyme required to digest 1 μg of Lambda DNA in 1 hour at in a total reaction volume of 50 μl. |
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Quality Control Assays : Ligation |
After 20-fold overdigestion with Nco I, 95% of the DNA fragments can be ligated and recut. |
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16-Hour Incubation |
A 50 μl reaction containing 1 μg of DNA and 40 Units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour. |
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Oligonucleotide Assay |
No detectable degradation of a single-stranded and double-stranded oligonucleotide was observed after incubation with 20 units of restriction endonuclease for 3 hours. |
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Enzyme Properties |
When using a buffer other than the optimal (Supplied) Buffer, it may be necessary to add more enzymes to achieve complete digestion |
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Reagents Supplied with Enzyme |
10X Buffer Y, BSA (10 mg/ml) |
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NcoI cuts hemi methylated site |
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