SARS-CoV-2 S Protein IgG ELISA Kit
- Catalog Number : E5039
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Introduction
This ELISA kit applies to the in vitro qualitative determination of SARS-CoV-2 Spike Protein IgG in serum or plasma from pneumonia patients infected with SARS-CoV-2.
General Information
| Reactivity | Human, SARS-COV-2, Virus |
|---|---|
| Application | ELISA |
| Assay principle | This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Spike protein IgG in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgG in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgG to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgG and HRP conjugated anti-human IgG will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgG exists in the tested samples or not. |
| Sample type | Serum,Plasma |
| Storage instruction | Store at 2-8 for 6 monthS |
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Item |
Specifications |
Storage |
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Micro ELISA Plate (Dismountable) |
8 wells ×12 strips |
2-8℃, 6 months |
|
Positive Control |
2 vials |
2-8℃, 6 months |
|
Negative Control |
2 vials |
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Sample & Control Diluent |
1 vial, 25 mL |
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Concentrated HRP Conjugated Mouse anti-human IgG（100×） |
1 vial, 120 μL |
2-8℃(Protect from light), 6 months |
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HRP Conjugate Diluent |
1 vial, 14 mL |
2-8℃,6 months |
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Concentrated Wash Buffer（25×） |
1 vial, 30 mL |
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Stop Solution |
1 vial, 10 mL |
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Substrate Reagent |
1 vial, 10 mL |
2-8℃(Protect from light), 6 months |
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Plate Sealer |
5 pieces |
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Product Description |
1 copy |
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Certificate of Analysis |
1 copy |
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