Assay Kits

Total Amino Acids (T-AA) Colorimetric Assay

  • Catalog Number : AS1002
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Introduction

This kit can be used to measure total amino acids (T-AA) content in serum, plasma, urine, animal and plant tissue samples.

General Information

Assay principle Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm.
Sensitivity 3.03 mmol/L
Detection range 3.64-100 mmol/L
Sample type Serum (plasma) ,urine and tissue
Storage instruction 2-8℃ , 6 months

PRINCIPLE of KIT

Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm.

Item

Component

Specification

Storage

Reagent 1

Powder A

Powder × 1 vial

2-8 , 6 months

Reagent 2

Acid Reagent

0.8 mL × 1 vial

2-8 , 6 months

Reagent 3

Powder B

Powder × 1 vial

2-8 , 6 months

Reagent 4

Powder C

Powder × 1 vial

2-8 , 6 months

Reagent 5

Protein Precipitator

15 mL × 1 vial

2-8 , 6 months

Microplate

96 wells

No requirement

Plate Sealer

2 pieces

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other.

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