|Storage instruction||Storage at -20 ℃|
1.No special equipment required.
|APPLICATION||-- Sticky end ligation -- Blunt end ligation -- TA ligation|
1. Prepare the ligation reaction mixture in a microcentrifuge tube. 5×Quick Ligase Buffer 2 µl Inserta X ng Vectorb ~50 ng T4 DNA Quick Ligase (1 U/µl) 1 µl Sterile distilled Water To 10 µl a. The molar ratio of the insert and vector should be among 3:1 to 8:1. b. For vector with blunt terminal, please perform the dephosphorylation of vector to prevent cyclization.
2. Incubate the reaction mixture 10-15 min at 25℃ for sticky end ligation and 20-40 min at 25℃ for blunt end ligation.
3. The reaction mixture can be used directly to transform.