Jak1(Phospho Tyr1034/1035) Rabbit pAb
- Catalog Number : AP1373
- Number : AP1373
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General Information
| Reactivity | Human, Mouse, Rat | |||||||||
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| Application | WB, IHC | |||||||||
| Host | Rabbit | |||||||||
| Clonality | Polyclonal | |||||||||
| Conjugate | Non-conjugated | |||||||||
| Isotype | IgG | |||||||||
| Immunogen | Synthesized phosho peptide around human Jak1(Tyr1034 and 1035) | |||||||||
| Molecular Weight | 132kD (Observed) | |||||||||
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | |||||||||
| Storage instruction | -15°C to -25°C/1 year(Do not lower than -25°C) | |||||||||
| Research topic | >>EGFR tyrosine kinase inhibitor resistance>>PI3K-Akt signaling pathway>>Necroptosis | |||||||||
| Alias | Tyrosine-protein kinase JAK1
Janus kinase 1 JAK-1 |
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| Recommended Dilution Ratio | WB 1:200-1000; IHC 1:50-300 | |||||||||
| Specificity | This antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1034 or Tyr1035.and dually phosphorylated at two sites | |||||||||
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. | |||||||||
| Gene Name | JAK1 JAK1A JAK1B | |||||||||
| Protein Name | Jak1(Tyr1034/1035) | |||||||||
| Database Link |
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| Background | This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]. | |||||||||
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Domain:Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.,Domain:The FERM domain mediates interaction with JAKMIP1.,Function:Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.,sequence Caution:Translation N-terminally extended.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.,similarity:Contains 1 FERM domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,subcellular location:Wholly intracellular, possibly membrane associated.,subunit:Interacts with IL31RA, JAKMIP1 and SHB.,tissue specificity:Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. | |||||||||
| Cellular Localization | Endomembrane system; Peripheral membrane protein. Wholly intracellular, possibly membrane associated. | |||||||||
| Tissue Expression | Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. | |||||||||
| Validation Data |
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
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