Jak1 Mouse mAb
- Catalog Number : AM1316
- Number : AM1316
-
Size:
Qty : - Price : Request 詢價
- Stock : Request
-
General Information
| Reactivity | Human, Rat | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Application | WB, IF, IP | |||||||||
| Host | Mouse | |||||||||
| Clonality | Monoclonal | |||||||||
| Conjugate | Non-conjugated | |||||||||
| Immunogen | Purified recombinant human Jak1 protein fragments expressed in E.coli. | |||||||||
| Molecular Weight | 130kD (Observed) | |||||||||
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | |||||||||
| Storage instruction | -15°C to -25°C/1 year(Do not lower than -25°C) | |||||||||
| Research topic | >>EGFR tyrosine kinase inhibitor resistance>>PI3K-Akt signaling pathway>>Necroptosis | |||||||||
| Alias | JAK 1
JAK 1A JAK 1B JAK-1 JAK1 JAK1_HUMAN JAK1A JAK1B Janus kinase 1 a protein tyrosine kinase Janus kinase 1 JTK3 Tyrosine protein kinase JAK 1 Tyrosine protein kinase JAK1 Tyrosine-protein kinase JAK1 |
|||||||||
| Recommended Dilution Ratio | WB 1:200-1000; ICC 1:200; IF 1:50-200 | |||||||||
| Specificity | This antibody detects endogenous levels of Jak1 and does not cross-react with related proteins. | |||||||||
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. | |||||||||
| Gene Name | Jak1 | |||||||||
| Database Link |
| |||||||||
| Background | This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]. | |||||||||
| Function | Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Domain:Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.,Domain:The FERM domain mediates interaction with JAKMIP1.,Function:Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.,sequence Caution:Translation N-terminally extended.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.,similarity:Contains 1 FERM domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,subcellular location:Wholly intracellular, possibly membrane associated.,subunit:Interacts with IL31RA, JAKMIP1 and SHB.,tissue specificity:Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. | |||||||||
| Cellular Localization | Endomembrane system; Peripheral membrane protein. Wholly intracellular, possibly membrane associated. | |||||||||
| Tissue Expression | Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue. | |||||||||
| Validation Data |
Western blot analysis of extracts from C6,Ramos,Jurkat and Hela cell lysates using Jak1 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa. | |||||||||
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Jak1 mouse mAb (dilution 1:200). | ||||||||||
Immunoprecipitation analysis of Hela cell lysates using Jak1 mouse mAb. |
.png)